DomenicoRonga et al. https:\/\/doi.org\/10.1016\/j.eja.2020.126091 Science Direct
\nPunti salienti
\nPrincipali effetti dell’ambiente sull’accumulo di gliadine e peptidi che attivano la celiachia.
\n\u2022 Il fattore genetico contribuisce all’accumulo di peptidi tossici.
\n\u2022 Gliadine e peptidi coinvolti nella celiachia dipendono anche da GDD e precipitazioni.
\n\u2022 La bassa evapotraspirazione diminuisce il livello di gliadine e peptidi coinvolti nella celiachia.
\n\u2022 La selezione per TP (peptidi tossici), IP (peptidi immunogenici), \u03b1-GliA2-6, \u03b3-Gli-5 potrebbe essere disancorata da GPC e GV
\nAbstract
\n\u201cThe impact of environment, genetic selection and their interactions on grain yield of durum wheat genotypes has been extensively studied; however, limited information is available for their influence on gluten quality associated with effects on the amount and composition of glutenins, gliadins and celiac disease (CD)-triggering peptides. In this study, a set of six commonly cultivated durum wheat genotypes were assessed in a multi-environment trial of eight site-year combinations in different Italian regions during two consecutive harvest years (2016 and 2017). While high-molecular-weight glutenin subunits (HMW-GS) were more stable between years, differences in total gluten proteins were mainly due to low-molecular-weight glutenin subunits (LMW-GS) and gliadins accumulation. After mass separation and quantification, two gliadin proteins \u2013 \u03b3-Gli-5 and \u03b1-GliA2-6 (41.1 and 33.8\u202fkDa, respectively) \u2013 were further studied together with toxic (TP) and immunogenic (IP) celiac disease-triggering peptides obtained via simulated gastrointestinal digestion. While TP accumulation was strongly influenced by the genotypes, IP showed marked variation in the different sites with significant genotype-by-year and genotype-by-site interaction. Specific agrometeorological variables (i.e. growing degree days and aridity index) in different growing phases showed a strong negative correlation with \u03b1-GliA2-6 and CD-associated peptides. Statistical analysis revealed that the level of gliadins and TP\/IP peptides were uncorrelated with grain protein content and yield (resa). The selection of plant materials with good technological properties but with a low content of CD-triggering peptides should combine with ad hoc environment (e.g. site) selection and management practices reducing crop evapotranspiration in the vegetative phase.\u201d
\nAbbreviazioni
\nAI aridity index
\nDtH days to heading<\/p>\n
GY grain yield
\nET0 total reference evapotranspiration
\nGDD growing degree days
\nGPC grain protein content
\nHMW-GS high-molecular-weight glutenin subunits
\nIP immunogenic peptides
\nLMW-GS low-molecular-weight glutenin subunits
\nRCBD randomized complete block design
\nSN spikes per unit area
\nTGW thousand kernel weight
\nTP toxic peptides
\nTW test<\/p>\n
Keywords
\nDurum wheat, Gluten protein, Gliadin fraction, immunogenic peptides, Environment effect<\/p>\n","protected":false},"excerpt":{"rendered":"
DomenicoRonga et al. https:\/\/doi.org\/10.1016\/j.eja.2020.126091 Science Direct Punti salienti Principali effetti dell’ambiente sull’accumulo di gliadine e peptidi che attivano la celiachia. \u2022 Il fattore genetico contribuisce all’accumulo di peptidi tossici. \u2022 Gliadine e peptidi coinvolti nella celiachia dipendono anche da GDD e precipitazioni. \u2022 La bassa evapotraspirazione diminuisce il livello di gliadine e peptidi coinvolti nella […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[71],"tags":[1626,1622,1618,1616,144,1620,1624],"class_list":["post-4541","post","type-post","status-publish","format-standard","hentry","category-articoli","tag-durum-wheat-it","tag-environment-effect","tag-gliadin-fraction","tag-gluten-protein","tag-grano-duro","tag-immunogenic-peptides","tag-peptidi-immunogenici"],"yoast_head":"\n