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Maturation and fermentation of a mixture of water, flour and yeasts and / or lactic bacteria

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Maturation and fermentation are just two of the many processes that take place within a dough: they are not the same thing and they must not be confused with each other. The general concept of “fermentation” is known and is quite well known and is understood as the complex of biological reactions involving the transformation of sugars as the lactic fermentation and the alcohol. During such metabolisms part of the sugars present in the dough are transformed by the yeast and by bacteria into acids, carbon dioxide, water, ethyl alcohol, energy etc.. and macroscopically result in a lowering of the pH value, in the production of aromatic substances and / or volatile, in gas production and in the increase of the dough mass. The term “maturation” on the other hand, refer to the complex of countless biochemical reactions that is the basis of cleavage or enzymatic breakdown of complex molecules into simpler molecules; macromolecules from complex to simple amino acids in the case of proteins, in the case of polysaccharides to monosaccharides. These reactions take long time, while the fermentation is much faster; the fermentation, however, can be slowed down very much if the dough is placed at rest in a low temperature (from 1 ° C to 4 ° C max). In this way we give the opportunity to the dough to mature; only to maturation occurred will choose to operate the gradual raising of the temperature in order to increase, to balance and optimize the fermentation process. Prolonghed fermentation and maturation also have an influence, not secondary, both on the shelf life both on the organoleptic characteristics of the finished product conferring, between the other, perfumes and flavors emphasized.

Deepening:

The fundamental importance of maturation

More digestible starch in the Monococcum wheat

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The amylose content in T. monococcum (23.3-28.6% of total starch) (Hidalgo et al .. 2014) is lower than durum wheat (30%) and soft wheat (35-43% ), suggesting that the amylose content increases as the number of genomes increases. It also has small starch granules (so-called B-type) in greater proportion than grown wheat. Even large starch granules (A-type) have a significantly lower diameter in the monococcus grain (13.2 μm) than durum wheat (15.3 μm) or soft wheat (23.8 μm) (Taddei et al., 2009) and all this contributes to the high digestibility of foods based on monococcus wheat (Taddei et al., 2009), consequently the surface per unit weight of the starch grain granules (764 μm) is greater than to soft wheat (550 μm), and therefore more rapidly hydrolyzed by amylases (Franco et al 1992). Not all starch is rapidly hydrolyzed during digestion, the fraction that resists digestion and absorption in the human small intestine is called “resistant starch” and has physiological effects comparable to those of dietary fiber. However, single-grain wheat has a low content (0.2%) in “resistant starch” when compared to common wheat (0.4% to 0.8%) (Abdel-Aal et al., 2008).

Tolerability of the monococcum wheat.

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A peculiar characteristic of this cereal is the high food tolerability. In the last few years numerous experimental evidences of the reduced toxicity of the monococcus wheat prolamins have been obtained. In particular, the prolamins of this cereal are not able to induce lesions in the intestinal mucosa of celiac patients (Auricchio et al., 1982; De Vincenzi et al., 1995; 1996) and to agglutinate K562 (S) cells, a in vitro tests strongly correlated with the “toxicity” of the prolaminic peptides. Furthermore, T. monococcum accessions of immuno-dominant sequences able to stimulate T lymphocytes have been identified (Molberg et al., 2005; Spaenij-Dekking et al., 2005; Zanini et al., 2013). Recently, Gianfrani et al. (2012) reported the results of a study on two monococcus wheat genotypes, Monlis and ID331, compared with the variety of tender wheat Sagittarius. However, while the proliferates of Sagittarius and Monlis, a variety of monococcus wheat free of ω-gliadins, are able to promote the proliferation of enterocytes in the crypts of the mucous membranes of celiac patients and to induce the synthesis of interliquine 15 (IL- 15) in intestinal villi enterocytes, ID321 prolamins, a monococcus wheat line containing only one ω-gliadin, show no effect. The results suggest that Monlis is able to activate innate immunity and promote the synthesis of interleukin 15 (IL-15), a key molecule in the induction of adaptive immunity, while ID331 does not seem capable of eliciting this type of immune response. All this is in agreement with the observation that the prolamins of the Monlis variety and other monococcus wheat genotypes without ω-gliadin behave like the prolamins of soft wheat in their ability to agglutinate K562 (S) cells and alter the intestinal epithelium. These rare toxic genotypes of monococcus wheat (<2%) differ from the others due to the peculiarity of not producing ω-gliadins, in which sequences able to counteract the toxicity of the other prolamins seem to be present. Although monococcus wheat proteins show reduced cytoxicity towards intestinal cells, the presence of immune-dominant epitopes precludes their use in the celiac diet.
On the other hand, considering that the incidence and severity of celiac disease depends on the quantity and the harmfulness of the prolamins and that some monococcus wheat genotypes have a high bread making quality coupled with absence of cytotoxicity and reduced immunogenicity, it is expected that use of monococcus flours in the diet of the general population, in which there is a high percentage of individuals genetically predisposed to celiac disease but not yet celiac, can help to contain the spread of this form of food intolerance. This suggests that the monococcus wheat, recently reported in cultivation in Italy by researchers of the Council for Research and Experimentation in Agriculture (CRA) of Rome and San Angelo Lodigiano, will play an important role in the prevention of celiac disease, both directly in the form of bread and pasta both indirectly as a model species for the study of the role of innate immunity in the onset of celiac disease. FROM: The new frontiers of food technologies and the celiac disease Norberto Pogna, Laura Gazza (2013). Volume 212, 1 December 2016, Pages 537-542 Further confirmations were highlighted by the research: Protective effects of ID331 Triticum monococcum gliadin on in vitro models of the intestinal epithelium. Giuseppe Jacomino et al. 2016. Highlights: • ID331 gliadins do not enhance permeability and do not induce zonulin release. • ID331 gliadins do not trigger cytotoxicity or cytoskeleton reorganization. • ID331 gastrointestinal digestion releases ω (105-123) bioactive peptide. • ω (105-123) exerts a protective action against the toxicity induced by T. aestivum. Abstract A growing interest in developing new strategies due to coeliac disease. In the current study, we investigate the biological effects of ID331 Triticum monococcum gliadin-derived peptides in human Caco-2 intestinal epithelial cells. Triticum aestivum gliadin derived peptides were used as a positive control. The effects on epithelial permeability, zonulin release, viability, and cytoskeleton reorganization were investigated. Our findings confirmed that ID331 gliadin did not enhance permeability and did not induce zonulin release, cytotoxicity or cytoskeleton reorganization of Caco-2 cell monolayers. We also demonstrated that ID331 ω-gliadin and its derived peptide ω (105-123) exerted to protective action, mitigating the injury of Triticum aestivum gliadin on cell viability and cytoskeleton reorganization. These results may represent a new opportunity for the future development of innovative strategies to reduce gluten toxicity in the diet of patients with gluten intolerance.

Monococcum Wheat (einkorn)

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Monococcus wheat protein content, on average 15-18%, is higher than that of other cultivated cereals and has a nutritional value higher than that of common wheat and durum wheat. The studies carried out at the Research Unit for the Qualitative Valorisation of Cereals of the Council for Research and Experimentation in Agriculture (CRA-QCE) in the last ten years have allowed to identify many peculiar and nutritionally interesting aspects of the monococcus wheat. Among the characteristics that make it unique in the field of straw cereals we have (i) the high content of carotenoids, precursors of vitamin A and natural antioxidants, which is about 5 times that of soft wheat; (ii) the excellent availability of tocoli (vitamin E), which is about 50% greater than durum wheat and soft wheat; (iii) the high content in lipids (about 50% more than common wheat), with a clear prevalence of unsaturated fatty acids; (iv) the high percentage in ash and the high content in minerals (particularly interesting are zinc, iron and phosphorus) and (v) a content in fruits about 50-70% greater than soft wheat (Hidalgo and Brandolini, 2008) ). The monococcus wheat flour, almost impalpable, has a characteristic yellow color and is excellent for the production of biscuits, snakes, flakes and other bakery products (Brandolini et al., 2008; Pollini et al., 2013); there are also genotypes with an excellent attitude to bread-making (Saponaro et al., 1995; Borghi et al., 1996). Also the pastification quality is very high, both in terms of workability of the raw material and of the quality of the finished product: the spaghetti and the monococco wheat macaroni have a good resistance to cooking and a reduced loss of starch compared to those based on commercial groats. of durum wheat (Brandolini et al., 2008). Moreover T. monococcum possesses small-sized (so-called B-type) starch granules in proportion to the cultivated wheats.

Deepening:
1. Tolerability of the monococcum wheat
2. More digestible starch in the monococcum wheat