“Previous studies have documented that landraces and older wheat varieties contain more diverse gene combinations for prolamins (wheat proteins) in comparison to modern varieties (10, 11). Literature shows variations for speciﬁc gene sequences mainly in the epitopic regions of Glia-α9, Glia-α2, Glia-α20, and Glia-α in older landraces (9). In the last decade in the context of CD, the immunogenicity of T-cell speciﬁc epitopes has been bought to the forefront (9, 12). The immunogenic potential amongst diﬀerent hexaploid wheat varieties is variable; hence it is possible that there are breeding-induced diﬀerences in the presence and expression of T-cell stimulatory epitopes in modern varieties of wheat (13, 14). This raises the question of, whether there is any speciﬁc variety of wheat which is less immunogenic and can be used in breeding programs for developing a wheat genotype completely safe for consumption by patients suﬀering from CD”.
• The identiﬁcation of less/non-immunogenic wheat species is an important milestone that could help patients or even prevent CD.
• With the use of gluten-speciﬁc T-cells and PBMCs, wheat genotypes containing minimally harmful gluten sequences can be selected.